hplc

is likely misspelled.

has no English definition.

As an initialism HPLC

is high performance liquid chromatography or high pressure liquid chromatography.

In analytical chemistry|lang=en terms the difference between hplc and tlc

is that hplc is (analytical chemistry) high performance liquid chromatography or high pressure liquid chromatography while tlc is (analytical chemistry) thin layer chromatography.

As initialisms the difference between hplc and tlc

is that hplc is (analytical chemistry) high performance liquid chromatography or high pressure liquid chromatography while tlc is tender loving care.